caski human cervical carcinoma cells  (Korean Cell Line Bank)

Journal: Nature Genetics

Article Title: High-content CRISPR activation screens identify synthetically lethal RNA-based mechanisms to sensitize cancer cells to targeted T cell cytotoxicity

doi: 10.1038/s41588-026-02561-7

Figure Lengend Snippet: a , MYC ORF-based overexpression sensitizes cervical cancer cells to T cell cytotoxicity. The fraction of surviving HPV + cervical cancer cells ( y axis) in coculture with E7 TCR T cells (1:1, 48 h), is shown for CaSki cells transduced to express a control, BID or MYC ORF (mean ± s.d., n = 3 technical replicates per ORF). **** P < 0.001, ordinary one-way ANOVA, Dunnett’s multiple-comparison test. b , Fold-change in A375 cell viability after 24-h treatment with FasL (200 ng ml −1 ), shown for A375 cells with ORF-based overexpression of different sensitizing hits compared to A375 cells with a control ORF (mean ± s.d., n = 3–6 technical replicates per ORF). **** P < 0.0001, ** P < 0.01, ordinary one-way ANOVA, Dunnett’s multiple-comparison test, compared to control cells. c , UMAP of Perturb-seq data of control (NTC) and MYC CRISPRa cells, colored based on: (1) culture conditions, (2) sgRNA and MYC expression level, (3) the expression of the MYC GA signature and (4) the expression of the CRISPRa-resistance hit GAS7 (log 2 1p-transformed tp100k). d , Expression of MYC GA signature in control (NTC) cells and cells with MYC CRISPRa sgRNAs, further stratified based on MYC expression in monoculture (left) and coculture (right). The number of cells in each group is shown in parentheses ( n ). Boxplots: the middle line shows the median, the box edges show the 25th and 75th percentiles and the whiskers show the most extreme points that do not exceed ±1.5× the interquartile range (IQR). Further outliers are marked individually with circles (minima or maxima). **** P < 0.0001, one-tailed Student’s t -test.

Article Snippet: The cervical cancer cell line CaSki (ATCC, cat. no. CRL-1550) was cultured in Roswell Park Memorial Institute (RPMI) medium with GlutaMAX (Gibco, cat. no. 72400-047) supplemented with 10% FBS and 1× Pen–Strep.

Techniques: Over Expression, Control, Comparison, Expressing, Transformation Assay, One-tailed Test

Journal: Medicina

Article Title: Expression of Core Hippo Pathway Proteins in Cervical Cancer and Their Association with Clinicopathologic Parameters

doi: 10.3390/medicina61122134

Figure Lengend Snippet: YAP knockdown efficiency confirmed by RT-PCR in Caski cervical cancer cells. ( A ) Representative RT-PCR images from four independent experiments (#1–#4), demonstrating consistently reduced YAP mRNA expression in YAP-silenced (K.D) cells compared with negative control (N.C) cells. GAPDH served as the internal control and exhibited stable expression. Full-length, uncropped gels are provided in . ( B ) Relative YAP mRNA expression normalized to GAPDH, based on densitometric analysis using ImageJ. Data represent the mean ± SD from four independent experiments. YAP expression decreased by approximately 50% in the K.D group (*** p < 0.001).

Article Snippet: Caski human cervical carcinoma cells were obtained from the Korean Cell Line Bank and cultured in RPMI-1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin at 37 °C in a humidified atmosphere containing 5% CO 2 .

Techniques: Knockdown, Reverse Transcription Polymerase Chain Reaction, Expressing, Negative Control, Control

Journal: Medicina

Article Title: Expression of Core Hippo Pathway Proteins in Cervical Cancer and Their Association with Clinicopathologic Parameters

doi: 10.3390/medicina61122134

Figure Lengend Snippet: Western blot validation of YAP knockdown in Caski cervical cancer cells. ( A ) Representative blots from four independent experiments showing reduced YAP and P-YAP expression in the knockdown (K.D) group compared with the negative control (N.C), with GAPDH as the loading control. ( B ) Densitometric analysis demonstrating an approximately 50% decrease in YAP levels in the K.D group (*** p < 0.001). ( C ) Densitometric analysis showing an approximately 45% reduction in P-YAP levels in the K.D group (** p < 0.01). Data represent mean ± SD from four experiments.

Article Snippet: Caski human cervical carcinoma cells were obtained from the Korean Cell Line Bank and cultured in RPMI-1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin at 37 °C in a humidified atmosphere containing 5% CO 2 .

Techniques: Western Blot, Biomarker Discovery, Knockdown, Expressing, Negative Control, Control

Journal: Medicina

Article Title: Expression of Core Hippo Pathway Proteins in Cervical Cancer and Their Association with Clinicopathologic Parameters

doi: 10.3390/medicina61122134

Figure Lengend Snippet: Wound-healing assay demonstrating the effect of YAP knockdown on cell migration in Caski cervical cancer cells. ( A ) Representative wound-healing images of negative control (N.C) and YAP-silenced (K.D) cells captured at 0, 12, 24, and 48 h after scratch formation. Red dashed lines indicate the original wound boundaries. YAP-silenced cells demonstrated visibly slower wound closure compared with N.C cells. ( B ) Quantitative measurement of wound gap distance using ImageJ. Gap width was measured across three independent experiments, and values are presented as mean ± SD. Significant differences between groups were observed at 24 h and 48 h ( p < 0.01), indicating impaired migratory ability following YAP suppression.

Article Snippet: Caski human cervical carcinoma cells were obtained from the Korean Cell Line Bank and cultured in RPMI-1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin at 37 °C in a humidified atmosphere containing 5% CO 2 .

Techniques: Wound Healing Assay, Knockdown, Migration, Negative Control